Over a decade ago, Prof. Shinya Yamanaka and his team developed a method to generate induced pluripotent stem cells (iPSCs) from adult mouse fibroblasts. These cells were pluripotent and were able to generate any cell lineage. A year later they introduced the first human induced pluripotent stem cells that were reprogrammed from adult human fibroblasts. His discovery revolutionized the research of human disease, as we are now able to generate a replication of different cell types from human patients that have the exact same genetic modifications as the patient.
Our lab uses multiple differentiation techniques to derive different types of human neurons and other brain cells, as 2D cultures and 3D organoids, from patients with neurological disorders. We use electrophysiology (whole cell patch clamp, multi electrode arrays and imaging of calcium transients), computational modeling, combined with molecular and cellular methods to look for mechanisms underlying disease states of the human brain with the goal of improving treatment.
Laboratory of Precision Disease Modeling | Shani Stern